New gonostyli is highlighted into the red, and will become clearly noticed in newly mated queens

New gonostyli is highlighted into the red, and will become clearly noticed in newly mated queens

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New gonostyli is highlighted into the red, and will become clearly noticed in newly mated queens

Females genitalia out of Yards. fasciculata and you will actions having mating connect coupling. (A) Virgin female genitalia in the front glance at. (B) Male genitalia off pre-copula male, addressing the feminine genitalia. (C–E) Genital medicine, metasomal sterna S-VIII and you can S-IX. (F) Men genitalia doing new coupling of your plug: ventral view of vaginal capsule and you can metasomal sternum S-IX projecting outside of the muscles of your men of the a telescopic movement. (G) Men vaginal supplement detached out-of male’s human body, having knob valves brought about, gonostyli up against the alternative reputation in terms of the initial. (H) Metasomal sterna S-VIII and you may S-IX, in which sternum S-IX assumes its brand new standing when you look at the male’s instinct. (I) Mating connect in detail, demonstrating this new lateral compressing of gonocoxites. (J) The fresh genital chamber out of good blocked girls: the newest membranous part which includes the brand new mating plug affixed.

Throughout the non-Melipona species (F

longipes, S. aff. Postica, and you can P. minima), we observed strong penis regulators that have mediator curvature and you can a lower life expectancy spatha ( Figure step one)paring the entire morphology from manhood regulators and you may spatha regarding M. fasciculata to another analyzed species, i unearthed that each other Yards. flavolineata and you will Meters. seminigra possess slim penis regulators having reduced curvature. not, Yards. seminigra provides less valves than M. flavolineata, and you will a more successful spatha when compared to additional Melipona ( Shape 1).

Malfunction regarding hypothesized plugging steps

The last updates away from mating plugs in to the mated girls coordinated the latest updates of male’s instinct while in the copulation because of “J” direction and position not as much as female’s gut ( Contour 4C). Throughout physogastric queens of Yards. fasciculata, i observed new mating connect attached to freesnapmilfs promo code the membranous vaginal pouches by dick regulators, towards the spatha facial skin created to the the feminine ventral section, placed directly under the newest gonopore ( Shape 3J). I propose that this new mating plug apparatus is actually triggered in the event that male vaginal product try irreversibly protruded regarding male’s looks, which results in changed ranking of gonostyli, knob regulators, and you may gonocoxites.

The newest mating set of Meters. fasciculata, predicated on a video clip listing out-of mating regarding an associated kinds. All the way through: (A) men in-flight, looking to updates alone over the people; (B) arranged male, proving sets away from legs fitting along the woman’s muscles; (C) male increasing the abdomen of the ladies; (D) men leaving the feminine once profitable mating, which is, the brand new mating connect attachment. Throughout pieces, male wings was illustrated to help you represent the course during the mating, centered on Additional Video clips S1 .

This new mating pair of Meters. fasciculata, centered on videos record out of mating from an associated kinds. From top to bottom: (A) male in flight, looking to updates in itself across the people; (B) arranged male, indicating sets regarding foot installing across the female’s human body; (C) male enhancing the abdomen of females; (D) male leaving the female immediately after effective mating, that’s, the newest mating connect accessory. Throughout parts, male wings try represented to depict the way during the mating, centered on Second Video S1 .

According to the review between sleep state out-of genital products and you may brought about state out-of mating plugs, its useful morphology, along with films suggestions into mating behavior regarding a directly relevant variety ( Second Films S1 ), we suggest that triggering of mating plug takes place in 5 hypothetical steps ( Figures step three and you will cuatro):

Shortly after immobilizing the female body, the male inserts their metasomal sternum S-VIII underneath the ladies tergum T-VI, training they ( Figure 4C).

Since female genital chamber is open ( Profile 3A), a man gonostyli joins new woman’s vaginal area. Once positioning the newest sternum S-VIII, a man metasomal sternum S-IX protrudes out of the male’s human body towards the the brand new woman’s genital chamber and the vaginal pill, of the good telescopic path ( Contour 3F). This new installation out-of men vaginal capsule on women vaginal chamber is not with the newest gonostyli, and that disperse backward inside the processes, leftover beyond your women genital chamber ( Contour 3G).

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